The Quant-iT PicoGreen dsDNA. • Specific for dsDNA in the presence of RNA. Rapidly detect as little as 0. This assay is designed for use with fluorescence microplate readers. This protocol is a simplified and condensed. quant it picogreen dsdna assay kit reagents. The micrococcal nuclease treatment used for fragmentation in some selected ChIP methods will generate. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267DNA concentration was measured using a spectrometer with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. According to the obtained results, the assay presents a linear correlation in a wide range of 0. This protocol is a simplified and c. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). This protocol is a simplified and condensed version of. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). Quant-iT OliGreen ssDNA Assay Kits contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. The HS (high sensitivity) assays quantitate the lowest levels of analyte, the BR (broad range) assays do so across a wider range, and the RNA XR. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. Picogreen assays are compatible with most microplate readers and fluorometers. The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures. When 5–500 pg salmon dsDNA or 3–1000 pg control dsDNA (calf thymus dsDNA standard) is added to AccuClear a linear response is observed (Fig. Picogreen assays are compatible with most microplate readers and fluorometers. 5) prepared from 20x. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. • Suitable for use in 96- and 384-well plate formats. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2. The Qubit 1X dsDNA BR Standards includes standards at two concentrations, 0 and 100 ng/µL. 0 ml) of the aqueous working solution of Quant-iT™ PicoGreen dsDNA Reagent was added to each cell sample, which was incubated for 10 min at 37 °C in the dark. Q33120) • Quant-iT dsDNA Assay Kit, broad range (Cat. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 05 to consider statistical significance. Pico Green dsDNA Assay 2 03/04/2019The assay was performed according to the initial MPO-DNA complex ELISA protocol, but blocking buffer, sample diluent and detection antibody diluent were replaced by an in-house blocking buffer. lambda DNA standard 100ng/uL (in kit) PicoGreen reagent (in kit) 20x TE buffer (in kit) HPLC water; PicoGreen Prep. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence. 5 ng/µL. 5). 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 1 mL kit. 2–100 ng, the fluorescence signal is linear with DNA. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. 2. Sample fluorescence was. ZERO BIAS . 3. 99. high-range. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. The assay is linear over three orders of magnitude and has little sequence dependence. 95 mL TE for the . 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 5 ng/mkl – 100 mg/ml. This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA. Le réactif et les kits de quantification d’ADNdb PicoGreen sont parfaits pour les dosages PCR, les échantillons de biopuces, les dosages de lésions de l’ADN, les dosages d’activité enzymatique, la quantification d’ADN génomique, la mesure de l’ADNdb dans des mélanges complexes et la quantification d’ADN viral. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Rapidly detect as little as 0. • Specific for dsDNA in the presence of RNA. If your are using plastic cuvettes or assay plates, reduce this problem by including 0. low-range. Bioz Stars score: 86/100, based on 1 PubMed citations. Quant-iT PicoGreen dsDNA Assay Kit 를 사용하면, ssDNA 와 RNA 그리고 free nucleotide 가 혼재되어 있는 시료에서도 dsDNA 만을 선택적으로 측정할 수 있으며, 25 pg /ml 의 매우 적은 양까지도 검출할 수 있습니다. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays*Selection Guide for the Qubit and Quant-iT Assay Kits—Table 8. Quant-iT™ PicoGreen™ dsDNA Assay KitInvitrogen - Thermo FisherCatalog #P11496 Quant-iT™PicoGreen® dsDNA reagent is dissolved in dimethylsulfoxide (DMSO), which freezes below 19 °C. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. 125 to 100 ng/mL. 86100 stars. 10 x 100 µL. DNA content was then determined using the Quant-iT PicoGreen dsDNA assay kit per manufacturer’s instructions. Catalog Number. Calibrator MPO concentration was quantified with a commercially available assay (Human Myeloperoxidase Quantikine ELISA Kit, Bio-Techne, Minneapolis, MN) according to the manufacturer’s recommendations. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 2023-11. The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. Per Well Volume 150 uL/well. Reagent Search & Data Services Menu Reagent Search. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Rapidly detect as little as 0. PCR products were quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (Bio-Tek, FLx800), and then mixed according to the amount of data required for each sample. Catalog number: P7581. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of. Rapidly detect as little as 0. The The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. Quantifications using the UVette require half the amount ofCell viability was measured using a WST-8 assay (Cell Counting Kit-8, Dojindo). LC3, and dsDNA, or α-synuclein, cathepsin D and dsDNA, Picogreen (1/10000, Quant-iT PicoGreen dsDNA Assay Kit. , QuantIt PicoGreen dsDNA assay). 実験してみた – PicoGreenをプレートリーダーで使用してみた. PicoGreen dsDNA quantitation reagents are. Rapidly detect as little as 0. Unit Size. HS Assay Kit or Quant-iT™ PicoGreen® dsDNA Assay Kit. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 3. 文件. P7589. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific,. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. PicoGreen dsDNA quantitation reagent is a highly sensitive fluorescent nucleic acid stain which is used to quantitate double-stranded DNA. The PicoGreen assay has high specificity for dsDNA versus RNA or ssDNA, and is. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. Quant-iT™ PicoGreen dsDNA Assay Kit (Life Technologies; Cat. 99 to $1999. 作成者 LatB Staff 01. The range of linearity for DNA concentration measurements. In case dsDNA standards are available only the quantification reagent can be used. Product Type. • Suitable for use in 96- and 384-well plate formats. Table 3. Description. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267dsDNA in the presence of equimolar concentrations of ssDNA and RNA with minimal effect on the quantitative results. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Dynamic range and sensitivity of the Quant-iTTM PicoGreen® dsDNA assay. Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). The assay is highly selective for double- stranded DNA over RNA, and in the. Qubit® dsDNA HS Assay Kit. Not for use in diagnostic procedures. Product Type. Q33130). Q33120). Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). dsDNA만을 선택적으로 정량. PicoGreen®試薬アッセイは、蛍光を基にした迅速かつ簡単な手法により、dsDNAを正確に定量することができます。. 50 pg to 2 µg. PicoGreen Solution must be diluted fresh before each assay run. 710 citations – Kit [P7589] Quant-iT™ PicoGreen™ dsDNA Assay Kit by Thermo Scientific. 5 µL of each extracted sample was used for Picogreen analysis. Picogreen assays are compatible with most microplate readers and fluorometers. The Qubit dsDNA HS and BR Assay Kits are accurate from 10 pg/L to 100 ng/uL, and 100 pg/L to 1,000 ng/L, respectively. 1 mL kit. Each sample was amplified in triplicate, and the triplicate amplicons were pooled and purified by gel extraction and quantified using Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, P7589). Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. quant it picogreen dsdna assay kit reagents. The DeNovix dsDNA Broad Range Assay is ideal for measuring sample concentrations ranging from 0. Thermo Fisher quant it picogreen dsdna quantitation assay kit. Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 0). • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. To perform fluorometric quantification of cfDNA quantity Quant-iT PicoGreen dsDNA Assay Kit (Thermofisher), was used, following the manufacturer’s instructions. 3 Q33120 39. Briefly, 2 µL of sample was diluted in 498 µL TE. PicoGreen dsDNA quantitation reagents are. Description. ru by: 1. Product Type. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. After a 5-minute. The PicoGreen is sticking to the sides of the cuvette or assay plate. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. Picogreen assays are compatible with most microplate readers and fluorometers. 20 and 2. PicoGreen dsDNA quantitation reagents are. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. Therefore, fluorescence spectroscopy using PicoGreen is more useful than UV spectroscopy for evaluating the template activity of DNA for PCR. For ds DNA can be used kits below: Quant-iT™ PicoGreen ® dsDNA Assay Kit (Invitrogen P7589); Qubit® dsDNA Broad Range Assay Kit (Invitrogen Q32850);. quant it picogreen dsdna quantitation assay kit - by Bioz Stars , 2023-09. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Figure 2. HepG2 cell number standard curve determined using the PicoGreen® assay (Quant-iTTM PicoGreen® dsDNA reagent kit, Invitrogen), HepG2 cells were harvested from conventional 2D T75 culture flasks and counted using a haemocytometer and Trypan Blue dye exclusion. 2009;49(4):997–1002. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT PicoGreen dsDNA Assay Kit a. Coleen - 06 Oct. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. Quantification and normalization of double stranded DNA with minimal set-up time. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Rapidly detect as little as 0. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. It is specific for dsDNA in the presence of equimolar amounts of RNA. Picogreen assays are compatible with most microplate readers and fluorometers. 0 fluorometer and Quant-iT PicoGreen dsDNA. • Suitable for use in 96- and 384-well plate formats. Description. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Rapidly detect as little as 0. Quant-IT dsDNA Assay Kit, high sensitivity Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target: dsDNA: dsDNA: dsDNA: Quantitation range: 0. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Three kits covering a wide range of dsDNA concentrations. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. 使用 Quant-iT Picogreen dsDNA 定量试剂盒和 dsDNA 试剂在较宽的动态范围内实现精准的 dsDNA 浓度测定。. a) 16. Rapidly detect as little as 0. Regulations & Guidance; Manuals 0 0. The Quant-iTTM dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Product Type. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. '. The Quant-iT™ PicoGreen® dsDNA reagent is a proprietary, asymmetrical cyanine dye. Triplicate 10 μL samples of λ DNA (Ο), E. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. io |. Qubit DNA and RNA assays are available with different quantification ranges. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. Unit Size. g. 10pg/µl vs. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Buy from Supplier. Catalog Number. As little as 2. Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer, BMG LABTECH, 12/2014. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. For Research Use. Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Picogreen assay kit (Invitrogen, P7589) a. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Prepared DNA concentration of the samples was 250 ng/ mL. For a standard. ZERO BIAS - scores, article reviews, protocol conditions and moreSelectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Prepare only the amount of 1:25 diluted PicoGreen required for that run. • Suitable for use in 96- and 384-well plate formats. No. NET formation quantification was performed using a MPO-DNA PicoGreen assay according to the manufacturer’s instructions (Quant-iT PicoGreen dsDNA Assay kit, Invitrogen). • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Reagent. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Quant-iT PicoGreen dsDNA Assay Kit. Rapidly detect as little as 0. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. 线，利用这一曲线并根据经验检测样本的浓度。Qubit dsDNA HS Assay可用于测量DNA浓度在10pg/µL 至100ng/µL 之间 的样本。超过此浓度范围的样本必须经过稀释然后进行重新 测试，以确保它们的结果落在参考标准的线性范围内。Qubit dsDNA HS Assay具有高度的准确. Quant It Picogreen Dsdna Assay Kit Manual - 114,697 . Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitQuant-iT™ PicoGreen® dsDNA Reagent and Kits | 4 Experimental Protoco l DNA Standard Curve 1. For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Cloning, expression and proteins purification. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesIn this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific, Waltham, MA, USA). Table 2 summarizes results of the. 7. Quant-iTTM PicoGreen® Assay Reagent 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Los ensayos Picogreen son compatibles con la mayoría de los lectores de microplacas y fluorómetros. 005% Triton X-100, Tween-20, or Brij. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Bioz Stars score: 86/100, based on 1 PubMed citations. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. We recommend using Quant-IT Picogreen as a high-sensitivity assay to measure DNA concentration in that range . Serial dilutions were made to give appropriate cell, seeding numbers. 1X dsDNA assay (%) 100 17. Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. 99. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. 2 to 100 ng) Assay Kit were used with a Qubit 3. RESULTS The OT-2 Constructs Highly Uniform LibrariesThermo Fisher double stranded dna quant it picogreen dsdna assay kit Double Stranded Dna Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Quant-iT dsDNA BR buffer (Component B) 250 ml c. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. For example, if the amount of the linear dsDNA library available is 20 ng, the linear dsDNA library input should be 10 ng, and the linear dsDNA library concentration should be at least 0. Data were expressed as mean ± standard deviation (SD) of n = 3 unless otherwise specified. The assay is linear over three orders of magnitude and has little sequence. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. Note: The 20X TE buffer (Component B) included in the PicoGreen dsDNA Quantitation Kit is certified to be nucleic acid-free and DNase-free. 5. n = 3. 0 mL to 200 uL to fit a 96-well microplate format. Rapidly detect as little as 0. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. High tolerance for contaminants. The kit contains. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitlimit consume only 2 picograms of dsDNA per measure-ment. Accuracy of the Qubit dsDNA HS assay compared to the Quant-iT PicoGreen dsDNA assay plotted on two different types of curves. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Product Description. AccuBlue® RNA Quantitation Kit Details. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 5mL of TE x 5uL PicoReagent. Prepare the 1x TE buffer by diluting the 20x TE concentrate 1:20 by deionized water. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers. Highly selective for dsDNA over ssDNA or RNA. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. For Research Use Only. 2. The original sequencing data. The normalized DNA was aliquoted into multiple tubes at 50µL volume. In addition, the concentration of DNA obtained from the 1860 tomato samples was measured fluorometrically using Quant-iT™ PicoGreen™ dsDNA Assay Kit (hereafter PicoGreen, Thermo Scientific, Waltham, MA, USA) and a 96-wells plate reader VICTOR3 1420 (PerkinElmer, Waltham, MA, USA) equipped with an excitation filter F485 and. 2. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Average 86 stars, based on 1. After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Picogreen assays are compatible with most microplate readers and fluorometers. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 1–100 ng: Ex / Em (nm) 502 / 523: 510 / 527: 502 / 523: Components: dsDNA HS fluorescent reagent, dilution buffer and standards RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. 2 to 100 ng) Assay Kit were used with a Qubit 3. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Instead of 2 mL, only 1 mL measurement volume was used. Quantify nucleic acids and proteins using four user-selectable fluorescent. クイックオーダー. using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). Price from $9. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Helixyte™ Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. PicoGreen® Assay Supplies Equipment: − NanoDrop 3300 Fluorospectrometer − 2uL pipettor (low retention nuclease free pipette tips) Materials:Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. Quant It. The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. The Quant-iT™ dsDNA High-Sensitivity Kit is designed for assaying samples containing 0. Picogreen assays are compatible with most microplate readers and fluorometers. 実験してみた – PicoGreenをプレートリーダーで使用してみた. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. Figure 2. 04 corresponds to 2 μ g/mL dsDNA solution. P7589. PicoGreen dsDNA Assay Kit. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Natural cures with herbs are. High tolerance for contaminants. Quant-iT dsDNA BR buffer (Component B) 250 ml c. a)Use 110 sample number per plate. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Quant-iT ™ 1X dsDNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33232 Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267 Quant-iT ™ DNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33120 Quant-iT ™ DNA Assay Kit, Broad-Range 4 ng−1 μg 1,000 reactions Q33130 Quant-iT ™The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read.